Adapter Removal Tutorial

Welcome to the Adapter Removal tutorial

This tutorial covers usage of the Adapter Removal tool for all versions of the small RNA Workbench past 2.5

If you wish to follow the tutorial yourself, please download the files found here and follow the video tutorial.

Alternatively just watch the video below to get an idea of how to use the sRNA Workbench to trim adapters from your raw sequence data.

Trimming adapters from FASTQ data

Data used in the tutorial:


(found in the TutorialDataV3 download in: TutorialData/FASTQ)

Download Video:
Closed Format: “MP4”
Open Format: “OGG”

  • Prasad

    hi i am using command line for adapter trimming. Every thing is working fine except i am unable to save the discarded reads which is there in the GUI. Any suggestions

    • The sRNA Workbench

      Hi Prasad,

      That option is not available from the CLI as yet. If it helps I can add it easily enough? Let me know and I will put it on the to-do list!


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A suite of tools for analysing micro RNA and other small RNA data from High-Throughput Sequencing devices