Quality Check, Normalisation and Differential Expression Tutorial

Welcome to the Quality Check, Normalisation and Differential Expression Workflow tutorial

This tutorial covers usage of the Quality Check Workflow from all current builds of Version 4.0 of the software and above.

If you wish to follow the tutorial yourself, please download the files found here and follow the video tutorial.

Alternatively just watch the video below to get an idea of how to use the sRNA Workbench to report on the quality of your biological samples, how to normalise your data, annotate your sequences and identify differentially expressed reads across your experiment

Quality Check, Normalise and Differential Expression

Data used in the tutorial

(found in the TutorialDataV4 download in: TutorialData/FASTA/RNAOME/Sample1/QC)

SRR1297338_na_0.1.fasta

SRR1297339_na_0.1.fasta

(found in the TutorialDataV4 download in: TutorialData/FASTA/RNAOME/Sample2/QC)

SRR1297341_na_0.1.fasta

SRR1297342_na_0.1.fasta

(found in the TutorialDataV4 download in: TutorialData/FASTA/GENEOME)

TAIR10_chr_all.fas

(found in the TutorialDataV4 download in: TutorialData/GFF)

TAIR10_GFF3_genes.gff

A suite of tools for analysing micro RNA and other small RNA data from High-Throughput Sequencing devices