Offset Review

Choosing an appropriate Offset value

To begin this calculation users must first select the normalisation they are going to use for their data from the side menu and then hit continue workflow

This node shows users a curve representing how diverged each abundance level is from a uniform strand bias (in small RNA loci).

The minimum of the curve represents the abundance level that contains the most noise.

Therefore, users need to adjust the slider to smooth the curve (shown here in blue) to best fit the trend of the data

Users are shown an image similar to this based on their data
Users are shown an image similar to this based on their data

A suite of tools for analysing micro RNA and other small RNA data from High-Throughput Sequencing devices