Choosing an appropriate Offset value
To begin this calculation users must first select the normalisation they are going to use for their data from the side menu and then hit continue workflow
This node shows users a curve representing how diverged each abundance level is from a uniform strand bias (in small RNA loci).
The minimum of the curve represents the abundance level that contains the most noise.
Therefore, users need to adjust the slider to smooth the curve (shown here in blue) to best fit the trend of the data